An analysis of water samples surrounding swine farms in Timis County – A practical guide

Authors: Crina Laura Moșneang1, Ordodi V.L.,2 Cristina R.T.1                                                                                   Affiliations: 1) USAMVB, Faculty of Veterinary Medicine Timisoara,                                                                                   2) UMF Timișoara, Institute of Imunophisiology – County Hospital Timișoara

The most important role in biological soil pollution is allocated to the untreated waste water used to ground’s fertirigation from livestock farms, and in particular of swine units. Applying of arbitrary measures, and national and European legislation’s non-compliance are main factors that often makes from this issue a public health problem by the great impact it can generate and create in large agglomerations and animals. The diluted manures are able to affect the quality of the environment mainly by: nitrous oxide, ammonia, methane, hydrogen sulphide, volatile organic compounds, etc. and they, being administered in soils, may cause epizootic and epidemiological aspects and also those relating to environmental protection. In this respect it rise the need for all livestock farms to apply appropriate measures for certain manure treatment, different to species of animals and depending on the collection and discharge systems used. This paper is an original research work and it intends to be also a practical guide to follow for those interested in field research of environmental pollution. There are presented current investigation methodologies of water’s quality from swine farms vicinity in Timis County. In four chapters are presented: primary water analysis methodology, the determination of chlorides, nitrates and phosphates for each substance being presented methodology, kits and reagents necessary specific results and their interpretation and conclusions for each study. The last chapter was allocated to the description of the potentially polluting compounds determination by GC-MS technique.

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A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances

Author: Violeta Giugiu                                                                                                                       Affiliation: Romvac Company S.A., Bucharest

The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substances in veterinary formulation. This formulation was submitted to accelerated degradation studies under acidic, alkaline and oxidative conditions, exposure to light and thermal stability. The separation of furazolidone, oxytetracycline and degradation products was achieved on BDS Hypersil C18 (250mmx4.6mm, i.d. 5 µm particle size) with gradient mobile phase containing methanol and 80 mM dipotassium phosphate pH 7,5 (20/80). The flow rate was 1.0 mL/min and detection was set at 254 nm, at 25 °C. The developed method was validated with respect to linearity, limits of detection and quantification, specificity, accuracy, and precision.

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An alternative approach to cat’s epilepsy treatment – Case report

Authors: Liliana Cărpinișan1, Laura Rațiu2, J. Degi1, Alina Ghișe1                                                       Affiliations: 1) Faculty of Veterinary Medicine Timișoara                                                                                                    2) “Bassy Veterinary Clinics” Timișoara

Angel, a female British Shorthair cat, would be euthanized at the recommendation of three veterinarians, after she manifested epileptic seizures starting from seven months old. Due to her owner and one veterinarian love and effort, she well passed through many seizures and a non-regenerative anemia episode and she survived for five years from the onset of the disease. The mineral-vitamin supplements improved the life quality for four years and the homeopathic therapy successfully removed the seizures for one more year.

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HPLC method for simultaneous determination of oxytocin and clorobutanol in injectable solutions

Authors: Ana Csuma, Despina Niţă, Ana Cişmileanu, Luciana Paraschiv                                             Affiliation: S.N. Pasteur Institute S.A., Bucharest

The objective of this work was to establish and validate a HPLC method with UV detection for simultaneous determination of oxytocin and clorobutanol in veterinary injectable formulations. The method is based on European Pharmacopoeia monograph for oxytocin concentrated solution. Chromatographic separation was achieved on a Syn Chropack RP100 C18 column (250 x 4,6mm, 5 μm) with a mobile phase consisting of solutia A: sodium dihydrogen phosphate buffer 0,13 M and solution B: acetonitrile-water (1:1, v/v) with gradient elution (30 % B for 1 min, 30 % B to 60 % B in 30 min, return to initial concentration and echilibration for 15 min before the following injection), at a flowrate of 1 ml/min and detection at 220 nm. The retention times for oxytocin and clorobutanol were about 15 min and 26 min respectively. The degradation products of oxytocin eluted at retention times smaller than 14 min. The resolution between oxytocin and the nearest impurity fulfilled the USP monograph requirements of at least 1,5. The linearity of the method has been settled from 2,5 UI/ml to 20 UI/ml for oxytocin and 1,25 mg/ml  to 10 mg/ml for clorobutanol. In these ranges the corelation coefficients were higher than 0,9950. The method allows the separation of oxytocin from degradation products and clorobutanol and could be applicable to quality control of injectable products containing oxytocin and clorobutanol. The method was validated in terms of selectivity, linearity, precision and accuracy.

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Report regarding the microbiological quality control of the veterinary pharmaceutical products during 2012

Authors: Simona Sturzu, Daniela Tirsinoaga, Ioana Tihulca, Mariana Dumitrache, Alina Draghici     Affiliation: Institute for Control of Veterinary Biological Products and Medicines, Bucharest

The microbiological contamination of pharmaceutical products could have major consequences on the physical – chemical characteristics, safety and efficiency of the medicinal products and also on the out of specification products recall-associated costs. Quality control of the pharmaceutical products should be a major objective for both the pharmaceutical industry as well as for the official quality control laboratories and the final results of these tests should provide most accurate data regarding the safety and the efficiency of the controlled pharmaceutical products. This report is presenting the results which were obtained in 2012 in the Microbiological Control Laboratory following the quality control of the products according to their pharmaceutical form and therapeutic class.

About the use of nano-therapeutic means in medicine

Authors: Andreia Chirilă and Romeo T. Cristina                                                                           Affiliation: Faculty of Veterinary Medicine Timișoara

Nanobiotehnology is a relatively new field of research, being the interface between the life sciences and nanotechnology. In this area where the work dimensions are between 1 nm and 100 nm the recovery of biomolecules’ quality and the processes involved it is proposed, in the development of materials or devices with certain medical activity. This bibliographic approach proposes a first foray into nano-therapeutic resources use in the medical field. Are presented sintheticaly the main nanomaterials, their properties and potential applications in nanomedicine, magnetic fluids, their synthezis and stabilization as well as recent advances in this topic.

Pharmacotherapy of hyperthyroidism in cats

Authors: Andreea Pap and Romeo T. Cristina                                                                               Affiliation: Faculty of Veterinary Medicine Timișoara

Currently, the feline hyperthyroidism possesses three types of treatment that includes medication, surgery and radioactive therapie. The success of these therapies consists in obtaining favorable results, by inhibiting thyroid hormone secretion, reducing clinical signs, improving the live’s quality and by absence of side effects of administered medication. One of these three therapies that fulfill these conditions and considered ideal for clinicians is the radiation therapy. Choosing this therapy depends not only on the availability of radioiodine licensed clinics or the presence of a skilled surgeon but also on the patient’s clinical condition, especially when a cardiovascular or renal disease is present in this review paper.

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