Studies on hematological parameters in broiler chicken treated with Amoxidem 50%

Authors: Turcu D.1, Mariana Oporanu1 P. Grigorescu1, M. Roman2,

Affiliation: 1 Faculty of Veterinary Medicine, Spiru Haret University, Bucharest                                                                  2 S.C. Delos Impex 96 S.R.L., Bucharest

Hematological response was studied in broiler chicken body as a consequence of treatment with Amoxidem 50% soluble powder. Under investigation were two batches of broiler chicken: control batch A and experimental batch B who received Amoxidem 50% soluble powder 46mg/Kg weight/day. Blood sampling was performed 7 days after treatment. The data were statistically analyzed using Student-Fisher method. The number of red blood cells showed no changes in statistical terms, the difference between batches being insignificant (p

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Determination of streptomycin residues in honey

Authors: Monica Cristina Cara1, Simion Gheorghita1, Mirabela Panfiloiu2

Affiliation: 1 Sanitary-Veterinary and Food Safety Directorate of Timis,                                                                               2 S.C. Antarctica S.R.L. Timisoara, Sanmihaiul Roman, Romania

Honey was the first sweet substance used in human food as a precursor sugar cane or beet. Honey production by bees is a complex process of transformation, from harvesting and ending with a striking honeycomb cells. Immediately after extraction, honey is always clear and liquid. This is due to the high content of fructose, which makes some items, such as locust always remain liquid. If the glucose is higher than that of fructose, honey will crystallize at temperatures below 18°C. Honey is easily susceptible to heat, so they should be stored at room temperature. Bees, like all living organisms, can become ill with various diseases. These, by mortality that occur among individuals of the colony, thereby reducing the number of bees and bee families are emptied and become unproductive. In many cases, it appears significant damage resulting in large losses for beekeepers. Prevention, detection and treatment of diseases with antibiotics is an extremely important factor in ensuring the health of bee.

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Virucidal properties of DEO-SEPT upon Vaccinia virus through quantitative suspension test

Authors: Danes M.1, Carmen Moisoiu2, Mihaela Scripcariu3, Rodica Moise2, Doina Danes4, Doina Surugiu2.

Affiliation: 1 SN Institutul Pasteur SA, Bucuresti                                                                                                             2 SC Pasteur Filipesti                                                                                                                                   3 ICBMV Bucuresti                                                                                                                                       4 USAMVB Bucuresti, Facultatea de Medicina Veterinara

Antiseptics and disinfectants are used extensively in hospital sand other health care settings for a variety of topical and hard-surface applications. In particular, they are an essential part of infection control practices and aid in the prevention of nosocomial infection. The virucidal efect of the DEO-SEPT product was demonstrated on the vaccinia virus strain Elstree (Essen University Origin) which has been passaged 10 times in GMK-AH1 (green monkey kidney cell line), three times in HeLa cells and five time in Vero cells (monkey kidney cell line). Evaluation of the effectiveness was done in a quantitative suspension test according with DVV (German Association of the Control of Virus Disease) and RKI (Robert Koch Institute) guideline at the 0.5%, 1% and 2% concentration. The contact time was 5, 15, 30 and 60 minutes into the present of the control product based on formaldehyde. The results showed the 99.9% inactivation of the initial virus titer (means >4log10 of the initial titer) at 2% after 5 minutes.

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The antihypertensive effect of amlodipine in cats

Authors: D. Morar, T. Mot, C. Falcă, Cristina Petruse, Petruta Lavinia Galbenu                                       Affiliation: USAMVB Timisoara, Faculty of Veterinary Medicine

The purpose of the study was to evaluate the effect of amlodipine on blood pressure and renal function in cats with arterial hypertension secondary to chronic renal failure. The research was conducted on 11 cats, aged between 7 and 14.5 years, diagnosed with arterial hypertension secondary to chronic renal failure. Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MBP) and pulse rate were determined by oscillometric method, before and after 7, 30 or 120 days of treatment with amlodipine. At the beginning of treatment, all cats were receiving 0.625 mg amlodipine once daily and after 7 days of treatment, in five cats, the dose was increased to 1.25 mg amlodipine, once daily. Before amlodipine administration the mean values of SBP/DBP were 175 ± 13.2 mmHg/119 ± 7.2 mmHg and after 30 days of treatment, the mean values of the SBP/DBP were reduced by 27.9/25.4 mmHg (p

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An another point of view on side effects of antifungal compounds used in veterinary medicine

Authors: Carmen Lidia Chitescu1, Anca Nicolau2

Affiliation: 1 S.C. Pasteur, Filipesti Branch,                                                                                                                     2 University Dunarea de jos, Galati, Romania

The release of pharmaceuticals into environment has become an increasing concern in recent years. Fungi are part of the microbial flora of many animals, humans and foodstuffs, and some species can cause disease. An antimycotic or antifungal product is one that is used in the treatment of fungal infections. Even at low concentrations, antifungals exert an action against micro-organisms and exhibit selective toxicity towards them. The use of antimicrobials selects for resistant populations of micro-organisms. Development of resistance to antifungals is an increasing problem in veterinary and human medicine.

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Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate

Author: Elena Gabriela Oltean                                                                                                     Affiliation: ROMVAC Company SA, Bucuresti

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of praziquantel and pyrantel pamoate in tablet dosage forms of TOTAL. HPLC separation was carried out by reversed phase chromatography Betasil C18 (250 mm x 4,6 mm i.d.; 5 μm particle size), held at 25°C respective Kromasil 60-5SIL. (250 mm x 4,6 mm i.d.; 5 μm particle size), held at 25°C. The mobile phase consisted of acetonitrile/distilled water (60/ 40 v/ v), run at flow rate of 1 mL/ min and with UV detection at 210 nm, respective acetonitrile / 0,1% phosphoric acid aq. (60/40 v/v), run at flow rate of 1 mL/ min and with UV detection at 240 nm. Method validation investigated parameters such as linearity (r2 = 0,9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of TOTAL tablets.

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The validation of the Analytical method (HPLC), use for identification and assay of the pharmaceutical active ingredient, colistine sulphate and the finished product Colidem 50 – hydrosoluble powder, in SC DELOS impex ‘96 SRL

Authors: Maria Neagu, Cristina Marinescu, Carmen Popescu                                                         Affiliation: SC DELOS IMPEX ’96 SRL, Bucuresti

In SC DELOS IMPEX ’96 SRL the quality of the active pharmaceutical ingredient (API) for the finished product Colidem 50 – hydrosoluble powder is make according to European Pharmacopoeia, curent edition. The method for analysis use in this purpose is the compendial method „Colistine sulphate” in E.P. in current edition and represent a optimized variant, developed and validated „in house”.The parameters which was included in the methodology validation for chromatographic method are the follow: selectivity/specificity, linearity, range of linearity, limit of detection and limit of quantification, precision (repeatability-intra day, inter-day reproducibility), accuracy, robustness, stability solutions and system suitability.

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Small therapy guide in swine’s diseases

Authors: Cristina T. Romeo1, Olimpia Ines Lupu (Paută)1, Iliuț Coman2

Affiliation: 1 Facultatea De Medicină Veterinară Timișoara
                 2 DSVSCA Sibiu

The paper is intended as a useful guide practicing veterinarian in the material being presented in a synthetic way the major prophylactic and therapeutic means in the main groups of pig diseases: suinopathies, bacterioses, viral and parasitic diseases.

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