Activity of an nutritional supplement in lamb’s natural infection with Cryptosporidium spp.

Authors: Imre K., Dărăbus Gh., Ilie M.S., Ionela Hotea, Mirela Imre                                                   Affiliation: USAMVB Timișoara, Facultatea de Medicina Veterinara

In this paper are presented the results of a test for eficiency of one nutritional supplement as a source of electrolites and glucose in natural infection with Cryptosporidium spp. at calves. During the treatment every calf was examined three times and one time a week after the treatment was finished. In every examination feces were collected to have a view of oochysts elimination. It was concluded that the product tested dose not reduce the elimination of Cryptosporidium spp. oocysts, but produces a clinical improvement, reducing the diarrhea and improving the general status of the animal.

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A spectrophotometrical analitic method elaboration for tinidazole’s quantity determination from tablets

Authors: Dan Dragos and Zoltan Szabadai                                                                                     Affiliation: Universitatea de Medicină si Farmacie „Victor Babes” Timisoara, Facultatea de Farmacie

The main inconvenience of analytical determination of Tinidazole in pharmaceutical formulations consists in the presence of ingredients, frequently with unknown identity. In order to overcome the complications of methods based on selective separations, a spectrophotometrical method for Tinidazole assay was performed and tested. By appropriate derivatization of the analythe, a photometrically estimable Schiff base is obtained, with absorption in the visible region where the interference of ingredients is negligible. The linearity of analytic signal was checked out in methanol solution in the 2-14 mg/l concentration domain. The method exhibit a good reproducibility: in a 8-fold repetition the main standard deviation is 0.38 % of the average of individual values.

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Enterococcus faecium strains characterization through polymorphism study of VNTR loci

Authors: F. Pastramă1, Virgilia Popa1, C. Stoica1, Irina Codită2, Vasilica Ungureanu2, Monica Ghită2,                          N. Catană3, Daniela Botus1, C. Belteghi1, G. Răpuntean4

Affiliation: 1 S.N. Institutul Pasteur S.A. Bucuresti                                                                                                          2 Institutul National de Cercetare Dezvoltare pentru Microbiologie si Imunologie CANTACUZINO,                          3 USAMVB Timisoara, Facultatea de Medicina Veterinară                                                                                    4 USAMV Cluj-Napoca, Facultatea de Medicină Veterinară

Enterococci are commensally bacteria of the gastrointestinal and female genital tract in humans and some mammals and birds, and one of the significant causes of hospital-acquired infections, especially in immuno-compromised patients. Genetic fingerprinting (DNA fingerprinting) is a tool for identifying, marking and prevention of infectious agents dissemination. SSR (short sequence repeat) are known to suffer frequent variations in the number of repetitive units. MLVA (multiple locus variable number tandem repeats analysis) is a variant of genetic fingerprinting, in epidemiological studies on the pathogenetic Enterococcus faecium. Our study included laboratory Enterococcus faecium strains or isolated from clinical cases or from the environment (2003-2008). All analyzed strains of Enterococcus faecium were sensitive to vancomycin, except BM4147, and resistant to oxacilin. Strains isolated from the birds’ samples have shown a smaller resistance profile than those of human origin. 33 Enterococus faecium strains were analyzed by PCR amplification. 27 MT (VNTR profiles) were obtained: six in the case of the strains isolated from birds, 15 in the case of the strains isolated form humans, 4 in the case of the collection strains and 2 in the case of the strains isolated from water samples. Among the strains isolated from humans and those isolated from animals, identical profiles were not recorded. Within the strains isolated from clinical cases, and those isolated from birds, circulating genotypes were noted, which can be considered as epidemical. The strains used as probiotics proved to be different from those circulating in birds. All MLVA profiles codes compared with those published on line in the UMC Utrecht database proved to be different. Results obtained in this study support the usefulness of the polymorphic VNTR analysis, as genetic marker, in epidemiological investigations.

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Avisalmvac: evaluation studies of stability and toxicity

Authors: Daniela Botus, Virgilia Popa, E. Bucur, E. Caplan, M. Pirvulescu, C. Belteghi, F. Pastrama Affiliation: SN Institutul Pasteur SA

In Pasteur Institute laboratories there was developed AVISALMVAC, a vaccine against avian Salmonella, a biological product that contains S. enteritidis and S. typhimurium bacterin, with oil adjuvant. This paper presents the results of the studies regarding the stability and toxicity evaluation of this vaccine stored under conditions recommended by the manufacturer (2-80°C) at the end of the period of validity. The vaccine stability was assessed by serological and histopathological analysis of samples from SPF chickens vaccinated with the product at the end of the period of validity. The study of Avisalmvac toxicity was carried out by inoculation of the product or its components on Vero cell monolayer, and the effects were microscopically recorded or by MTT test, applied at 6 days post-inoculation. Antibody titers recorded at 2 and 3 weeks post vaccination demonstrated the vaccine ability (used after an year since manufacture) to induce synthesis of specific antibodies and therefore, the product stability was proven. Histopathological examinations carried out on samples taken at 18 days post vaccination from the vaccination site (skeletal muscle and skin) and spleen, did not show any lesions associated to vaccination with Avisalmvac. The cytotoxicity analysis made by inoculating the vaccine or its components on Vero cell monolayer and the microscopic examination did not record visible cytopathic effects for any vaccine dilutions or vaccine components. The cell metabolism evaluation by MTT assay made at 6 days after vaccine/vaccine components inoculation on Vero monolayer, shown the ability of the vaccine and oil adjuvant to stimulate cell metabolism, and a certain degree of toxicity/inhibition of dehydrogenase metabolism associated to one of emulsifier but at dilutions higher than those used in the vaccine
formula.

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Drugs’ maximal residual limits determination methodology and waiting period’s establishing, in the light of E.U. regulations

Author: Romeo T. Cristina                                                                                                             Affiliation: USAMVB Timișoara, Facultatea de Medicina Veterinara

Communitary concepts related to the drug residues assumption is essential to Romanias’ full integration amongst the countries where alimentary security and human consumers’ security have had become national policy. In this respect a concise enumeration of E.U. basic legislative notions, with the specific terminology and of technical premises presentation, it is proposed in the aim of Communitary veterinary medicinal products’ residues evaluation as well the MRL’s determination phases in the light of 2377/90 EU Regulation’s understanding.
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Ground principles of animal’s blood medication

Author: Romeo T. Cristina                                                                                                           Affiliation: USAMVB Timișoara, Facultatea de Medicina Veterinara

In the paper ground principles of blood medication in animals are enumerated. There are presented animal’s blood type groups (antigens and iso anticorps in dog and cat), auto transfusion in animals, blood substituents (free haemoglobins, fluorocarbons), anaemias’ therapy (erithro and granulopoetins, blood’s therapy specific steroids and vitamins, iron and his derivatives, cobalt), haemostasys and haemostatics and ant coagulation substances.

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Oestrogene-mimetic isoflavones’ pharmacokinetics and pharmacodynamics

Authors: Anca Dragomirescu, Dan Dragos, Mihaela Andoni                                                           Affiliation: Universitatea de Medicinã si Farmacie „Victor Babes”, Timisoara, Facultatea de Farmacie

Genisteine is the most abundant and the most studied estrogen-mimetic izoflavone. It’s chemical formula is: 4′,5,7-trihidroxyisoflavone. It has also estrogen-modulated properties by its binding ability to the beta type estrogen receptor. Genisteine presents the following farmacodinamic effects: antiaterogen effect, prevention of estrogen-dependent cancers, especially breast cancer, prevention of skin aging body, osteoprogen effect, prevention of osteoporosis at the menopauses women. Despite all these real benefits, there are also many adverse effects, registered both in humans and animals. Thus, the sheep feeding with some Fabaceae species, containing estrogen-mimetic isoflavones were stopped thir reproductive function (isoflavones acted as an oral contraceptive. In humans, phytoestroges influence is still under evaluation, being suspected effects such as cerebral involution – via abusive apoptosis – or disturbance in hormonal status, in male children. All these are added to already known allergies, caused by soy proteins.

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Diabetes mellitus in dogs and cats: diagnosis and therapy

Authors: Mot T., Cristina Petruse, Morar D., Simiz F., Ciulan V.                                                             Affiliation: USAMVB Timisoara, Facultatea de Medicina Veterinara

Diabetes mellitus (DM) is a disease of humans and animals, which causes increased levels of blood sugar (glucose). Normally,glucose is brought into the cells by a hormone – insulin.The cells then metabolize glucose to make energy used for all functions of the body. Animals suffering from DM either lack insulin, or the cells cannot use the insulin that is there. As a result, blood glucose levels increase, and the cells have to use other substances for energy. When blood glucose levels become too high, glucose is found in the urine, causing increased frequency of urination and increased drinking. When blood glucose remains elevated over a period of time, other metabolic changes can occur, such as weight loss, acidosis, seizures, coma, blindness, cataracts, and nerve damage. Animals that are eating normally and not showing signs of illness may only require a blood or urine test to diagnose DM. Concurrent diseases (such as infection, Cushing’s disease, hyperthyroidism, pancreatitis, gastroenteritis, inflammatory bowel disease, hepatic lipidosis, or kidney disease) make diabetes more difficult to diagnose and manage. A complete blood screen and other specific tests may be recommended to obtain the diagnosis and baseline values for treatment and future monitoring. The treatment for diabetes in dogs is similar to the treatment for diabetes in humans, through diet and insulin therapy. Dogs and cats with DM
are usually treated with insulin. Insulin is a protein and, as such, not suitable for oral administration. Thus, it is administered once or several times daily by the subcutaneous route. Adjustment of the blood glucose concentration demands long hospital care, and subsequently the owner constantly has to keep a strict schedule
at home. In veterinary practice the main groups of oral antidiabetic (used in human medicine either) are: carbohydrate absorption inhibitors (e.g. acarbose); insulin sensitisers (biguanides such as metformin, thiazolidinedions, peroxisome proliferator-activated gamma receptor agonists).

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Anticoccidal therapy small guide

Author: Romeo T. Cristina                                                                                                                         Affiliation: USAMVB Timisoara, Faculty of Veterinary Medicine 

Being a major challenge, because of ingenious parasite’s survival strategies and treatments’ transience, anticoccidal therapy it is a major priority. In this respect, therapeutic conduct must be adjusted as well is possible on the most efficient drug groups find on the market. Paper brings concise information for the practitioners’ up to date, about the therapeutic groups highly efficient in fight against coccidian offensive, amongst these recalling groups of: benzene-acetonitrils, benzyl-purines, carbanilides, guanidines, dinitro benzamides, polyetheric ionofors, thiamine antagonists, pyridines, quinolones, quinazolines, anticoccidia sulphonamids and symmetrical triazinones.

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