Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations

Authors: Violeta Tauber (Giugiu), Viorica Chiurciu
Affiliation: Romvac Company S.A.

An isocratic reversed phase high performance liquid chromatographic method with DAD detection was developed for the analysis of metronidazole, oxytetracycline and furazolidone. The mobile phase consisted of pH 2.5 phosphate buffer solution, methanol and acetonitrile (70 : 18 : 12). The UV detection was carried out at 264 nm, and the flow rate was 1.100 mL / min. The separation was carried out on a Nucleosil C18 column, 5 μm 250 mm x 4.6 mm, which was maintained at 25°C. This method was validated by system suitability parameters, linearity, limits of detection and quantification, precision and accuracy.

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Development and validation of a RP-HPLC method for the quantization studies of metronidazole in tablets and powders dosage forms

Authors: Elena Gabriela Oltean, A. Nica                                                                                       Affiliation: Romvac Company SA, Bucuresti

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of metronidazole in tablets and powders. HPLC separation was carried out by reversed phase chromatography on Kromasil C18 (250 mm x 4.6 mm i.e.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of methanol/ 0.1% phosphoric acid aq. (20/80 v/v), with a flow rate of 1 ml/ min and with UV detection at 317 nm. In order to validate the method, the following parameters have been investigated: linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceutical compound in tablets and powders. This paper aimed to develop and validate an HPLC sensitive applicable method to determine the quantity of metronidazole in tablets and powders, contributing to the quality and safety control of these types of pharmaceutical preparations.

full text (in English)

Development and validation of a RP- HPLC method for the quantittation studies of metronidazole and furazolidone from product Enteroguard M

Authors: Elena Gabriela Oltean1, Milea B.2

Affiliation: 1 Romvac Company SA,                                                                                                                                                                          2 Proanalysis Systems SRL

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole and furazolidone in tablet dosage forms of ENTEROGUARD M. HPLC separation was carried out by reversed phase chromatography Kromasil C18 (250 mm x 4,6 mm i.d.; 5 +m particle size), held at 25°C. The mobile phase consisted of methanol/ 0,1% phosphoric acid aq. (20/ 80 v/ v), run at flow rate of 1 mL/ min and with UV detection at 317 nm. Method validation investigated parameters such as linearity (r2 = 0. 9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of ENTEROGUARD M tablets.

 full text (in Romanian)