Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations

Authors: Violeta Tauber (Giugiu), Viorica Chiurciu
Affiliation: Romvac Company S.A.

An isocratic reversed phase high performance liquid chromatographic method with DAD detection was developed for the analysis of metronidazole, oxytetracycline and furazolidone. The mobile phase consisted of pH 2.5 phosphate buffer solution, methanol and acetonitrile (70 : 18 : 12). The UV detection was carried out at 264 nm, and the flow rate was 1.100 mL / min. The separation was carried out on a Nucleosil C18 column, 5 μm 250 mm x 4.6 mm, which was maintained at 25°C. This method was validated by system suitability parameters, linearity, limits of detection and quantification, precision and accuracy.

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Forced degradation of metronidazole, oxytetracycline and furazolidone studies using High Performance Liquid Chromatography

Authors: Violeta Tauber (Giugiu), Viorica Chiurciu
Affiliation: Romvac Company S.A.

The study describes the development of a HPLC-DAD method for the estimation of oxytetracycline and furazolidone in Enteroguard M – powder. The proposed method utilizes a Betasil C 18 column, (4.6 x 250 mm, 5 μm), at 300 °C, optimum mobile phase consisted of 40 mM phosphate buffer (pH = 7.5): methanol in gradient elution. The flow rate was optimized to 1mL / min and the detection was carried at 254 nm. The drug substance was exposed to temperature, light, oxidants, acid and basic hydrolysis, and the resultant samples were analyzed by the proposed method.

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A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances

Author: Violeta Giugiu                                                                                                                       Affiliation: Romvac Company S.A., Bucharest

The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substances in veterinary formulation. This formulation was submitted to accelerated degradation studies under acidic, alkaline and oxidative conditions, exposure to light and thermal stability. The separation of furazolidone, oxytetracycline and degradation products was achieved on BDS Hypersil C18 (250mmx4.6mm, i.d. 5 µm particle size) with gradient mobile phase containing methanol and 80 mM dipotassium phosphate pH 7,5 (20/80). The flow rate was 1.0 mL/min and detection was set at 254 nm, at 25 °C. The developed method was validated with respect to linearity, limits of detection and quantification, specificity, accuracy, and precision.

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Simultaneous determination of related substances of oxytetracycline in pharmaceutical semi solid form by Hight Performace Liquid Chromatography

Author: Violeta Giugiu                                                                                                                               Affiliation: Romvac Company S.A., Bucharest

Simultaneous determination of oxytetracycline, 4-epioxytetracycline, alpha-apooxytetracycline, tetracycline, beta-apooxytetracycline on C18 columns has been accomplished using a high performance liquid chromatographic method with UV detection. Separation was achieved on a Hypersil BDS RP-C18 column with a mobile phase consisted of acetonitrile-methanol-80mM dipotassium phosphate pH 7,5 at a flow rate of 0,7 mL min-1. Absorbance measurements were held at 254 nm.

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