Development and validation of a RP-HPLC method for the quantization studies of metronidazole in tablets and powders dosage forms

Authors: Elena Gabriela Oltean, A. Nica                                                                                       Affiliation: Romvac Company SA, Bucuresti

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of metronidazole in tablets and powders. HPLC separation was carried out by reversed phase chromatography on Kromasil C18 (250 mm x 4.6 mm i.e.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of methanol/ 0.1% phosphoric acid aq. (20/80 v/v), with a flow rate of 1 ml/ min and with UV detection at 317 nm. In order to validate the method, the following parameters have been investigated: linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceutical compound in tablets and powders. This paper aimed to develop and validate an HPLC sensitive applicable method to determine the quantity of metronidazole in tablets and powders, contributing to the quality and safety control of these types of pharmaceutical preparations.

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Development and validation of a RP-HPLC method for the quantization studies of ivermectin in solutions dosage forms

Authors: Elena Gabriela Oltean, A. Nica                                                                                     Affiliations: Romvac Company SA, Bucuresti

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of ivermectin in solutions. HPLC separation was carried out by reversed phase chromatography on KROMASIL C18 (150 mm x 4.6 mm i.e.; 5 μm particle size), kept in thermostat at 25°C. The mobile phase consisted of methanol/ water (90/ 10 v/ v), with a flow rate of 1 ml/ min and with UV detection at 254 nm. In order to validate the method, the following parameters have been investigated: linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceutical compound in solutions.

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Development and validation of a RP–HPLC method for the quantization studies of albendazole suspensions dosage forms of Rombendazol

Authors: Elena Gabriela Oltean, A. Nica                                                                                       Affiliation: Romvac Company SA, Bucuresti

An isocratic high–performance liquid chromatography (HPLC) procedure was developed for quantitative determination of albendazole in suspensions dosage forms of Rombendazol, HPLC separation was carried out by reversed phase chromatography KROMASIL C18 (150 mmx4.6 mm i.e.; 5 ìm particle size), held at 25°C. The mobile phase consisted of Methanol/Distilled Water (65/35 v/v), run at flow rate of 1.2 mL/ min and with UV detection at 308 nm. Method validation investigated parameters such as linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of suspensions dosage forms.

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Development and validation of a RP- HPLC method for the quantitation studies of bromadiolone in Ratitox F

Authors: Elena Gabriela Oltean, A. Nica                                                                                       Affiliation: Romvac Company SA, Bucuresti

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of bromadiolone (hydroxycoumarins) in Ratitox F product – rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 Hypersil C18 (250 mm x 4.6 mm i.e.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of methanol/ 0.1% aqueous solution phosphoric acid (90/ 10 v/ v), with a flow rate of 1 ml/ min and with UV detection at 265 nm. In order to validate the method, the following parameters have been investigated- linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of Ratitox F – rodenticide.

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Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate

Author: Elena Gabriela Oltean                                                                                                     Affiliation: ROMVAC Company SA, Bucuresti

An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of praziquantel and pyrantel pamoate in tablet dosage forms of TOTAL. HPLC separation was carried out by reversed phase chromatography Betasil C18 (250 mm x 4,6 mm i.d.; 5 μm particle size), held at 25°C respective Kromasil 60-5SIL. (250 mm x 4,6 mm i.d.; 5 μm particle size), held at 25°C. The mobile phase consisted of acetonitrile/distilled water (60/ 40 v/ v), run at flow rate of 1 mL/ min and with UV detection at 210 nm, respective acetonitrile / 0,1% phosphoric acid aq. (60/40 v/v), run at flow rate of 1 mL/ min and with UV detection at 240 nm. Method validation investigated parameters such as linearity (r2 = 0,9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of TOTAL tablets.

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