A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances

Author: Violeta Giugiu                                                                                                                       Affiliation: Romvac Company S.A., Bucharest

The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substances in veterinary formulation. This formulation was submitted to accelerated degradation studies under acidic, alkaline and oxidative conditions, exposure to light and thermal stability. The separation of furazolidone, oxytetracycline and degradation products was achieved on BDS Hypersil C18 (250mmx4.6mm, i.d. 5 µm particle size) with gradient mobile phase containing methanol and 80 mM dipotassium phosphate pH 7,5 (20/80). The flow rate was 1.0 mL/min and detection was set at 254 nm, at 25 °C. The developed method was validated with respect to linearity, limits of detection and quantification, specificity, accuracy, and precision.

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Simultaneous determination of related substances of oxytetracycline in pharmaceutical semi solid form by Hight Performace Liquid Chromatography

Author: Violeta Giugiu                                                                                                                               Affiliation: Romvac Company S.A., Bucharest

Simultaneous determination of oxytetracycline, 4-epioxytetracycline, alpha-apooxytetracycline, tetracycline, beta-apooxytetracycline on C18 columns has been accomplished using a high performance liquid chromatographic method with UV detection. Separation was achieved on a Hypersil BDS RP-C18 column with a mobile phase consisted of acetonitrile-methanol-80mM dipotassium phosphate pH 7,5 at a flow rate of 0,7 mL min-1. Absorbance measurements were held at 254 nm.

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